I’ve added the fungal spawn I got at Jess’s Intermediate Mushroom Growing workshop to coffee grounds from my favourite cafe. There are Elm and Yellow Oyster mushroom spawn and I found a bag of inoculated grain for White Button Mushrooms that was several years old.
The jars and grounds in the microwave for a good 15 minutes to ensure no opposition from other, unseen, competitors. 15 minutes was just a guess, but seemed more than enough. The jars were closed with tight fitting, plastic, lids to stop critters getting in and give the fungi a head start.
Then they were left for 5 days in a cool, dark, part of the laundry until the white fuzz of the mycelia could be seen in every jar, ensuring colonization. This ensured there was no transplant shock either.
I removed the plastic lids and replaced them with cheesecloth held on by rubber bands and moved them into the temporary greenhouse whyere it is warmer and humid.
Wish them luck!